Pci neo vector promega
Many Promega Vectors are reporter vectors containing various combinations of luciferase genes (firefly, Renilla, NanoLuc luciferase) with other genetic elements. The luminescence signal from these reporter vectors is used as an indicator of the activity of any gene/DNA element fused to the reporter.
Your time is valuable! Mammalian pCI-neo Mammalian Expression Vector. DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression. E1841. pAdVAntage™ Vector. When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation. E1711 The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells.
18.06.2021
Belgium). Restriction digestion of NS4B gblock and pCI-neo vector (Promega, Cat# E1841) was performed by using XhoI and XbaI restriction enzymes. Following restriction, NS4B gblock and pCI-neo vector were ligated using T4 DNA ligase (Prome ga). The ligation mix was transformed in electro- For the transfection of tumor cell lines with the pGL3 Luciferase Reporter Vector (Promega catalog #E1741) and pCI-neo Mammalian Expression Vector (Promega catalog #E1841) using the SuperFect Transfection Reagent from Qiagen (Catalog #301305).
Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide.
About the Flexi® Vector System. The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells.
The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression.
vector. pCI neo or pEF6/V5-His empty vector was added to adjust for total amount of DNA. After 24 h of transfection or a kit provided by Promega according to the Vector Type Resistance Marker Source Sequence Available; pCI-neo: Mammalian Expression: Neomycin: Ampicillin: Promega: Sign Up for Our Newsletter. Receive the This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells.
strands produced by the pCI Vector. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells.
DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression. E1841 pCI-neo vector (Promega) that had been digested with NheI and SalI. Purification of wt and K415A / K419A 2— Sf21 cells were prepared in a 1-liter spinner at a concentration of 1 10 6 cells/ml, into which the The pCI-neo Vector can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G-418. Return to this vector's summary.
About the Flexi® Vector System. The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells.
The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The pCI-neo Mammalian Expression Vector(a) carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells.
Following restriction, NS4B gblock and pCI-neo vector were ligated using T4 DNA ligase (Prome ga). The ligation mix was transformed in electro- For the transfection of tumor cell lines with the pGL3 Luciferase Reporter Vector (Promega catalog #E1741) and pCI-neo Mammalian Expression Vector (Promega catalog #E1841) using the SuperFect Transfection Reagent from Qiagen (Catalog #301305). 1. Plate 50,000 cells in 5 mL of the appropriate growth medium in a 60mm dish.
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The pHTC HaloTag® CMV-neo Vector is a simple multiple cloning site (MCS) plasmid for traditional cloning. It is designed for use with other HaloTag® C-terminal vectors for expression of C-terminal-tagged HaloTag® fusion proteins in mammalian cells.
.. The resulting vector was named as pCIn.hNGF. Belgium). Restriction digestion of NS4B gblock and pCI-neo vector (Promega, Cat# E1841) was performed by using XhoI and XbaI restriction enzymes.